Journal: Toxicology and applied pharmacology

Article Title: Cardiovascular alterations at different stages of hypertension development during ethanol consumption: time-course of vascular and autonomic changes.

doi: 10.1016/j.taap.2014.08.012

Figure Lengend Snippet: Fig. 2. (Left) Changes in mean arterial pressure (ΔMAP) evoked by increasing concentrations of the selective α1-adrenoceptor agonist phenylephrine in animals receiving water (control groups) or increasing concentration of ethanol in drinking water (first week: 5%, second week: 10%, third week: 20%, and fourth week 20% v/v). Circles represent the mean ± SEM. (Right) Maximal effect (Emax) and dose at 50% of the MAP range (ED50) for phenylephrine dose–response curves. The bars represent the mean ± SEM. *P b 0.05 vs respective control group, two- way ANOVA followed by Bonferroni's post hoc test.

Article Snippet: The membranes were blocked by incubation with blocking buffer (10%BSA in TBSt–1.6MNaCl and 0.2MTris, 0.1%Tween 20) for 2 h. After blocking, blots were incubated overnight at 4 °C with primary antibodies to α1a-adrenoceptor (1:1000 dilution; c-19, sc1477, Santa Cruz Biotechnology, Santa Cruz, CA, USA), β2-adrenoceptor (1:500 dilution; M-20, sc-570, Santa Cruz Biotechnology), and β-actin (1:10,000 dilution; 13E5, Rabbit mAB #4970, Cell Signaling Technology).

Techniques: Control, Concentration Assay

Journal: Toxicology and applied pharmacology

Article Title: Cardiovascular alterations at different stages of hypertension development during ethanol consumption: time-course of vascular and autonomic changes.

doi: 10.1016/j.taap.2014.08.012

Figure Lengend Snippet: Fig. 4. Time-course of relative protein levels (protein level/β-actin) of α1a- and β2-adrenoceptor in the mesenteric arterial bed (left) and aorta (right) in animals receiving water (control groups) or increasing concentration of ethanol in drinking water (first week: 5%, second week: 10%, third week: 20%, and fourth week 20% v/v). Circles represent the mean ± SEM. *P b 0.05 vs respective control group, two-way ANOVA followed by Bonferroni's post hoc test.

Article Snippet: The membranes were blocked by incubation with blocking buffer (10%BSA in TBSt–1.6MNaCl and 0.2MTris, 0.1%Tween 20) for 2 h. After blocking, blots were incubated overnight at 4 °C with primary antibodies to α1a-adrenoceptor (1:1000 dilution; c-19, sc1477, Santa Cruz Biotechnology, Santa Cruz, CA, USA), β2-adrenoceptor (1:500 dilution; M-20, sc-570, Santa Cruz Biotechnology), and β-actin (1:10,000 dilution; 13E5, Rabbit mAB #4970, Cell Signaling Technology).

Techniques: Control, Concentration Assay

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Primary alloproliferative TH1 response induced by immature plasmacytoid dendritic cells in collaboration with myeloid DCs.

doi: 10.1111/j.1600-6143.2005.01097.x

Figure Lengend Snippet: Figure 4: The phenotype of the responder T-cell population is similar in MDC and 5000PDC+100MDC allostimulatory conditions. After 5 days of culture, the alloproliferating CFSE-low T cells were stained with different mAbs to the indicated markers. (A) The expression of IL2Ra, IL12Rb1 and CD154 on proliferating T cells (gated on R1) is shown. The numbers indicate the percentage of cells in each quadrant. (B) Summary of the markers analyzed and detected (+ve) on responding CFSE-low T cells stimulated by 5000MDC (black bars) or 5000PDC+100MDC (white bars) (mean ± SD, n = 4). As most cells expressing the markers are CFSE low, the proportion is calculated based on the proliferating cells (contained in the R2 regions). (C) The time-dependent expression of IL12Rb2 on CFSE-low T cells was analyzed in three different culture experiments stimulated by (5000PDC+100MDC) (white symbols) or 5000MDCs alone (black symbols) for the indicated time period.

Article Snippet: The following murine monoclonal antibodies (mAbs) were used: Peridinin Chlorophyll Protein (PerCP)-labeled CD3 and HLA-DR; Fluorescein isothiocyanate (FITC)-labeled CD4 and CD45RA; Phycoerythrin (PE)-labeled CD11c, CD123, IL2Ra, CD154, CD45RO, IL12Rb1, IL12Rb2, IL18Ra (BD Biosciences Oxford, UK); TRI-Color® (TC)-labeled CD3, PE-labeled IL4, Allophycocyanin (APC)-labeled IFNc (Caltag Laboratories, Hamburg, Germany); FITC-labeled anti-BDCA2 (Miltenyi Biotec, Bergisch Gladbach, Germany); RPE-Cy5-labeled CD14, CD19 (Serotec Ltd, Kidlington, Oxford, UK).

Techniques: Staining, Expressing